Queen Bee stingers are not barbed, and because of this they can sting repeatedly without dying
“A virgin queen is a queen bee that has not mated with a drone. Virgins are intermediate in size between workers and mated, laying queens, and are much more active than the latter. They are hard to spot while inspecting a frame, because they run across the comb, climbing over worker bees if necessary, and may even take flight if sufficiently disturbed. Virgin queens can often be found clinging to the walls or corners of a hive during inspections.Virgin queens appear to have little queen pheromone and
often do not appear to be recognized as queens by the workers. A virgin queen in her first few hours after emergence can be placed into the entrance of any queenless hive or nuc and acceptance is usually very good, whereas a mated queen is usually recognized as a stranger and runs a high risk of being killed by the older workers.When a young virgin queen emerges from a queen cell, she will generally seek out virgin queen rivals and attempt to kill them. Virgin queens will quickly find and kill (by stinging) any other emerged virgin queen (or be dispatched themselves), as well as any unemerged queens. Queen cells that are opened on the side indicate that a virgin queen was likely killed by a rival virgin queen. When a colony remains in swarm mode after the prime swarm has left, the workers may prevent virgins from fighting and one or several virgins may go with after-swarms. Other
virgins may stay behind with the remnant of the hive. As many as 21 virgin queens have been counted in a single large swarm. When the after-swarm settles into a new home, the virgins will then resume normal behavior and fight to the death until only one remains. If the prime swarm has a virgin queen and the old queen, the old queen will usually be allowed to live. The old queen continues laying. Within a couple of weeks she will die a natural death and the former virgin, now mated, will take her place.Unlike the worker bees, the queen’s stinger is not barbed. The queen can sting repeatedly without dying.
Piping describes a noise made by virgin and mated queen bees during certain times of the virgin queens’ development. Fully developed virgin queens communicate through vibratory signals: “”quacking”” from virgin queens in their queen cells and “”tooting”” from queens free in the colony, collectively known as piping. A virgin queen may frequently pipe before she emerges from her cell and for a brief time afterwards. Mated queens may briefly pipe after being released in a hive.Piping is most common when there is more than one queen in a hive. It is postulated that the piping is a form of battle cry announcing to competing queens and the workers their willingness to fight. It may also be a signal to the worker bees which queen is the most worthwhile to support.The piping sound is a G♯ (aka A♭). The adult queen pipes for a two-second pulse followed by a series of quarter-second toots. The queens of Africanized bees produce more vigorous and frequent bouts of piping.”